Right here from the whole genome sequencing of 55 honey bees by design a top solution recombination chart from inside the honey bee, we discovered that crossovers try regarding the GC blogs, nucleotide range, and you may gene thickness. I together with confirmed the former idea you to definitely family genes indicated into the worker minds provides unusually highest CO rates. Our study hold the examine you to diversity of staff behavior, however resistant setting, try a motorist of the large crossing-more rates within the bees. We find no facts that the crossing-over rates are accompanied by a high NCO rates.

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Five territories of honeybees (Apis mellifera ligustica Spin) have been built-up of a beneficial bee farm when you look at the Zhenjiang, China. For every single colony contained you to definitely queen, all those drones, and you may hundreds of pros. Bees away from three territories have been selected to have whole genome sequencing.

The fresh new DNA of every personal is extracted using phenol/chloroform/isoamyl liquor method. To attenuate the possibility of bacterial contamination, brand new abdomens from bees were removed before DNA extraction. Regarding step three ?g away from DNA of for each shot were used to have whole genome resequencing since the left DNA is kept to possess PCR and you may Sanger sequencing. Structure of DNA libraries and you may Illumina sequencing was indeed performed https://datingranking.net/local-hookup/akron/ on BGI-Shenzhen. Into the temporary, paired-avoid sequencing libraries that have enter measurements of five hundred bp was basically built for each and every attempt depending on the maker’s rules. After that dos ? 100 bp matched-end checks out was indeed made for the IlluminaHiSEq 2000. New queens was indeed sequenced in the approximately 67? publicity an average of, drones from the approximately 35? publicity, and you will gurus in the everything 29? […]